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We performed an observational retrospective cohort study of symptomatic patients diagnosed with LGV in the STI clinic of the dermatology department of a public institution, Hospital Costa del Sol, Marbella, Spain. We examined all cases recorded between October and May The study population was fully screened for STIs according to risk practices, and serology test results were updated.

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Further, willingness to use a rectal enema for HIV prevention was assessed using an unbalanced item, which may have impacted participants to select a more positive attitude compared to them potentially having a neutral stance about their willingness. As new types of HIV prevention methods become available, our findings may help identify perceived barriers for their future use.

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